LOCALIZATION OF GLYCINE NEUROTRANSMITTER TRANSPORTER (GLYT2) REVEALS CORRELATION WITH THE DISTRIBUTION OF GLYCINE RECEPTOR

We studied by immunocytochemical localization, the glycine neurotransm itter transporter (GLYT2) in mouse brain, using polyclonal antibodies raised against recombinant N-terminus and loop fusion proteins. Wester n analysis and immunocytochemistry of mouse brain frozen sections reve aled caudal-rostral gradient of GLYT2 distribution with massive accumu lation in the spinal cord, brainstem, and less in the cerebellum. Immu noreactivity was detected in processes with varicosities but not cell bodies. A correlation was observed between the pattern we obtained and previously reported strychnine binding studies. The results indicate that GLYT2 is involved in the termination of glycine neurotransmission accompanying the glycine receptor at the classic inhibitory system in the hindbrain.