C. Gondolf et al., 2 MESENCHYMAL TUMOR-CELL LINES FOR THE ME ASUREMENT OF NATURAL-KILLER(NK) CELL-ACTIVITY IN THE PERIPHERAL-BLOOD OF DOGS, Berliner und Munchener Tierarztliche Wochenschrift, 108(2), 1995, pp. 47-50
The two mesenchymal cell lines K1 and K6 were established from round c
ell tumours located at the lips of two dogs. Both cell lines were char
acterized as being of myelomonocytic origin by morphological, cytochem
ical, immunocytochemical and functional criteria. In the (51)chromium
release assay (effector/target cell ratio 100/1) the K6-cell line reve
aled a mean cytotoxic sensitivity of 53.6% and thus showed a susceptib
ility similar to that of the epithelial CTAC line (57.8%). The K1-cell
line exhibited less cytotoxic activity (41%) when incubated for 14 h,
but showed results comparable to the K6-cell line, when the incubatio
n time was reduced to 8 h.