INTRACELLULAR GALACTOSE-OXIDASE FROM A MUTANT STRAIN DEFECTIVE IN ITSSECRETION

Authors
KITAMURA AE AMARAL D ZANCAN GT
Citation
Ae. Kitamura et al., INTRACELLULAR GALACTOSE-OXIDASE FROM A MUTANT STRAIN DEFECTIVE IN ITSSECRETION, Arquivos de biologia e tecnologia, 37(4), 1994, pp. 907-912
Citations number
NO
Categorie Soggetti
Biology
Journal title
Arquivos de biologia e tecnologia → ACNP
ISSN journal
03650979
Volume
37
Issue
4
Year of publication
1994
Pages
907 - 912
Database
ISI
SICI code
0365-0979(1994)37:4<907:IGFAMS>2.0.ZU;2-R
Abstract
Intracellular galactose oxidase (EC.1.1.3.9) was purified from a mutan t (DD09) of the original enzyme-producing fungal strain Dactylium dend roides. This mutant strain has an impairment in the enzyme secretion. The enzyme is a protein of 70kDa containing 4.5% of neutral sugar resi dues of which 73% are resistent to beta - elimination. Unlike the wild type,the enzyme showed a higher affinity for dihydroxyacetone than fo r galactose.