ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE

Authors
ITO H KOTAKE T MASAI M MIZUTA T ADACHI M
Citation
H. Ito et al., ESTABLISHMENT OF HUMAN BLADDER-CANCER CELL-LINE (HAMT-1) AND LONG-TERM SUBCULTURE IN NUDE-MICE, Oncology Reports, 2(2), 1995, pp. 221-226
Citations number
27
Categorie Soggetti
Oncology
Journal title
Oncology Reports → ACNP
ISSN journal
1021335X
Volume
2
Issue
2
Year of publication
1995
Pages
221 - 226
Database
ISI
SICI code
1021-335X(1995)2:2<221:EOHBC(>2.0.ZU;2-Z
Abstract
Human bladder cancer cell line (HAMT-1) was established from a transit ional cell carcinoma of the bladder (T4N3M0, grade 3) of a 61-year-old male by using a serum-free medium. HAMT-1 cells in serum-free medium were non-adhesive to the flask wall or to other cells. The cells grew in attached form by the addition of fetal calf serum. Heterotransplant ation of HAMT-1 cells into nude mice and the long-term subculture were successful. Tumor bearing nude mice showed an elevated serum tissue p olypeptide antigen as in the patient.