ASSOCIATION BETWEEN GENE ALTERATION AND DRUG-SENSITIVITY IN HUMAN LUNG-CARCINOMA CELL-LINES

The association between gene alterations (K-ras, p53, N-myc) and drug resistance (CDDP, CBDCA, MMC, Epi-ADM) was examined in 29 human lung c arcinoma cell lines using the in vitro MTT assay. There was no signifi cant difference in the IC50 values of four drugs between K-ras or p53 gene alteration-positive and -negative groups. However, two cell lines with N-myc amplification showed a higher resistance than those withou t N-myc amplification to all four drugs. This preliminary study sugges ts that K-ras or p53 gene alteration is probably not related to drug r esistance, but N-myc might be.