LOSS OF HETEROZYGOSITY ON THE SHORT ARM OF CHROMOSOME-17 IN UTERINE CERVICAL CARCINOMAS

DNA samples from 26 cervical carcinoma and normal tissue pairs were st udied by restriction fragment length polymorphism (RFLP) analysis to d etermine the Frequency of loss of heterozygosity (LOH) on 17p. Allelic loss in the p13.1 region of chromosome 17, known to contain the TP53 locus, was not detected in any of 10 informative cases. Instead, LOH w as detected on 17p13.3 in eight (40%) of 20 informative cases with at least one of two 17p13.3 markers. Examination of the intragenic region of p53 in the same samples using polymerase chain reaction (PCR)-RFLP analysis showed no LOH in the gene (none of Is informative individual s).