Sa. Igdoura et Cr. Morales, ROLE OF SULFATED GLYCOPROTEIN-1 (SGP-1) IN THE DISPOSAL OF RESIDUAL BODIES BY SERTOLI CELLS OF THE RAT, Molecular reproduction and development, 40(1), 1995, pp. 91-102
Sulfated glycoprotein-1 (SGP-1) is a polypeptide secreted by Sertoli c
ells in the rat. Sequence analysis revealed a 76% sequence similarity
with human prosaposin produced by various cell types. Human prosaposin
is a 70 kDa protein which is cleaved in the lysosomes into four 10-15
kDa polypeptides termed saposins A, B, C, and D. The function of lyso
somal saposins is to either solubilize certain membrane glycolipids or
to form complexes with lysosomal enzymes and/or their glycolipid subs
trate to facilitate their hydrolysis. The present investigation dealt
with the delivery of SGP-1 into the phagosomes of Sertoli cells; these
phagosomes contain the residual bodies which detach from the late spe
rmatids at the time of spermiation. Immunogold labeling with anti-SGP-
1 antibody was found over Sertoli cell lysosomes, but was absent from
phagosomes formed after phagocytosis of spermatid residual bodies in t
he apical Sertoli cell cytoplasm in stages VIII and early IX of the cy
cle of the seminiferous epithelium. The phagosomes found later in the
basal Sertoli cell cytoplasm in stages IX and X of the cycle became la
beled with the antibody as the components of the residual bodies rapid
ly underwent lysis and disappeared from the Sertoli cells. Sertoli cel
l lysosomes isolated by cell fractionation (estimated purity of 80%) w
ere found to contain a 65 kDa form of SGP-1 or prosaposin, as well as
the 15 kDa polypeptides or saposins. Thus, it appears that this unique
lysosomal form of SGP-1 reached the Sertoli cell phagosomes and that
their derived polypeptides, the saposins, must play a role in the hydr
olysis of membrane glycolipids found in phagocytosed residual bodies.
(C) 1995 Wiley-Liss, Inc.