CHARACTERIZATION OF NUCLEOSIDE PHOSPHOTRANSFERASE FROM LEISHMANIA-TROPICA

Authors
SHIN IS WATAYA Y
Citation
Is. Shin et Y. Wataya, CHARACTERIZATION OF NUCLEOSIDE PHOSPHOTRANSFERASE FROM LEISHMANIA-TROPICA, Nucleosides & nucleotides, 14(1-2), 1995, pp. 13-22
Citations number
18
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
14
Issue
1-2
Year of publication
1995
Pages
13 - 22
Database
ISI
SICI code
0732-8311(1995)14:1-2<13:CONPFL>2.0.ZU;2-Q
Abstract
Nucleoside phosphotransferase, a unique purine salvage enzyme of proto zoa Leishmania tropica, was partially purified. The purified sample wa s nucleosidase free. With this enzyme preparation, phosphorylation of 14 nucleosides involving 5 antileishmanial purine nucleoside analogs w as investigated. Apparent Km values of the enzyme for the 10 purine nu cleosides were in the range 5.7-8.4 mu M, and those for the 4 natural pyrimidine nucleosides were 14.0-16.9 mu M.