SYNTHESIS AND SUBSTRATE PROPERTIES OF THYMIDINE 5'-TRIPHOSPHATE ANALOGS WITH LARGE HYDROPHOBIC SUBSTITUENT GROUPS AT ALPHA-P ATOM

The new thymidine 5'-O-triphosphate analogs - thymidine 5'-0-alpha-phe nylphosphonyl-beta,gamma- diphosphate 4, 3'-fluoro-3'-deoxythymidine 5 '-O-alpha-phenylphosphonyl-beta ,gamma-diphosphate 5 and thymidine 5'- O-alpha-decylphosphonyl-beta,gamma-diphosphate 6 were synthesized. The ir substrate properties towards avian myeloblastosis virus and human i mmunodeficience virus reverse transcriptases, DNA polymerase beta from rat liver and terminal deoxynucleotidyltransferase from calf thymus w ere demonstrated. The mixture of Rp and Sp diastereomers of 4 was sepa rated by reversed phase HPLC, diastereomeric selectivity of the reacti on of DNA chain elongation was noted.