MOLECULAR-GENETIC MAPPING OF THE MOUSE MALE-STERILITY AND HISTOINCOMPATIBILITY (MSHI) MUTATION ON PROXIMAL CHROMOSOME-10

The recessive male sterility and histoincompatibility (mshi) mutation in the mouse generates pleiotropic effects on histocompatibility and m ale reproduction, while female mutants appear to be reproductively nor mal. We have mapped the mshi mutation to mouse Chromosome (Chr) 10 by analysis of 126 progeny from an intraspecific backcross. Our analysis both places the male sterility and histoincompatibility controlled by mshi within a 20-cM interval between the markers D10Mit51/D10Mit212 an d D10Mit170 and has allowed the ordering of several other microsatelli te markers on Chr 10 that were previously unresolved. The high-resolut ion backcross panel we describe should facilitate the isolation of mor e tightly linked probe sequences and, ultimately, the molecular identi fication of the gene or genes affected by this interesting mutation. ( C) 1997 Academic Press