CHARACTERIZATION OF A MUS-SPRETUS YAC THAT MAPS TO THE PSEUDOAUTOSOMAL REGION

A library, containing M. spretus DNA in a half-YAC vector, was made an d screened for clones hybridizing with an oligomer of the telomere hex amer TTAGGG. FISH to metaphase spreads of spleen cells showed hybridiz ation of clone YTY3 to the distal ends of both X and Y chromosomes, co nsistent with localization to the pseudoautosomal region (PAR). Recomb inational mapping in the BXD RI strains and an interspecies backcross, using a plasmid subclone and PCR primers from YTY3, showed linkage to distal Chr X. The restriction map of the YAC contains three NotI site s. Sequences similar to Mov15 lie close to the vector end of the clone , while the other end contains a telomere array that appears to be int erstitial within the PAR, suggesting that the insert represents a prox imal portion of the PAR. Sequences homologous to the clone are also pr esent at subtelomeric regions of autosomes 4, 9, and 13. (C) 1997 Acad emic Press