COMPARATIVE LINKAGE MAPPING OF HUMAN-CHROMOSOME-13 AND BOVINE CHROMOSOME-12

A comparative linkage map of human chromosome 13 and bovine chromosome 12 was constructed using eight polymorphic microsatellite markers ass ociated with six specific genes. Linkage of these was also examined re lative to five previously mapped anonymous microsatellite markers. Sev en gene-linked markers were developed from bovine large-insert genomic clones containing one of five genes of interest (serotonin receptor s ubtype 2, fms-related tyrosine kinase, coagulation factor 10, retinobl astoma susceptibility gene, collagen type IV alpha I), and one additio nal marker was developed from a microsatellite resident within an intr on of the bovine dopachrome tautomerase gene, Four of these loci were previously assigned to bovine chromosome 12 by analysis of a somatic c ell hybrid panel. This study provides linkage information for examinin g gene order in this conserved synteny group. The comparative linkage mapping results indicate that the q arm of human chromosome 13 is almo st entirely conserved in bovine chromosome 12. One intrachromosomal re arrangement was detected in this linkage group relative to human, and this rearrangement was confirmed by fluorescence in situ hybridization results. (C) 1997 Academic Press