W. Meyer et al., DEMONSTRATION OF CUTANEOUS ELASTIC FIBER ARRANGEMENT IN UNDAMAGED THICK SECTIONS BY CONFOCAL LASER-SCANNING MICROSCOPY, EJD. European journal of dermatology, 5(1), 1995, pp. 77-79
The study introduces a non-invasive method that demonstrates the elast
ic fibre network of the mammalian dermis in thick frozen sections, bas
ed on confocal laser scanning microscopy. This technique avoids dehydr
ation and shrinkage, so that the spatial relationships of elastic fibr
es are represented more true to life. This is best achieved by image p
rocessing, in particular by 3-D reconstruction of the basic structure
of the elastic system.