PRECLASSIFICATION OF PSEUDOMONAS-SYRINGAE PV AVELLANAE AS PSEUDOMONAS-AVELLANAE (SPEC NOV), THE BACTERIUM CAUSING CANKER OF HAZELNUT (CORYLUS-AVELLANA L)
Jd. Janse et al., PRECLASSIFICATION OF PSEUDOMONAS-SYRINGAE PV AVELLANAE AS PSEUDOMONAS-AVELLANAE (SPEC NOV), THE BACTERIUM CAUSING CANKER OF HAZELNUT (CORYLUS-AVELLANA L), Systematic and applied microbiology, 19(4), 1996, pp. 589-595
Fourteen Pseudomonas strains causing canker of hazelnut in Greece and
Italy were compared by analysis of plasmid DNA, whole cell fatty acids
, whole cell proteins, and restriction fragment patterns of 16S rRNA.
Moreover, fatty acid and protein profiles, 16S rRNA sequences and perc
entages of DNA similarity were compared with those of other Pseudomona
s bacteria, including P. aeruginosa, P. cichorii, P. corrugata, P. vir
idiflava, P. syringae pv. syringae, P. s. pv. porri, P. s. pv. phaseol
icola, P. s. pv. maculicola, P. s. subsp. savastanoi and P. fluorescen
s. The bacterium causing canker of hazelnut originally was named P. sy
ringae pv. avellanae on the basis of biochemical tests. Strains of the
hazelnut organism were very homogeneous, which was confirmed by our w
ork. Only in the number of plasmids, especially in Italian strains, so
me variation was observed. Fatty acid and protein profiles, as well as
16S rRNA sequence analysis and percentage of DNA-DNA hybridization wi
th other Pseudomonas strains indicated that the bacterium causing cank
er of hazelnut is very much apart from all pathovars of P. syringae re
sted, including pv. syringae. The strains were only very distantly rel
ated to P. syringae and other Pseudomonas species tested. All other Ps
eudomonas species tested were closer to P. syringae than the bacterium
causing canker of hazelnut. We therefore propose to reclassify P. syr
ingae pv. avellanae into P. avellanae.