Nippostrongylus brasiliensis (Nb) infection of mice induces IL-4 produ
cing CD4(+) T cells which stimulate polyclonal IgE and IgG1 production
, providing a model system to study IL-4 action on a cells in vivo. B
cell la expression and the proportion of IL-2R beta positive B cells w
ere increased in Nb-inoculated mice, and B cells from these mice respo
nded to IL-2 by prompt and marked cell growth. Injection of anti-IL-4
1 day after Nb inoculation substantially inhibited these responses, in
dicating that they were largely IL-4 dependent. Thus IL-4 acted as a p
olyclonal a cell activator in vivo and caused a cells to develop into
IL-2 responsive cells. Furthermore, injection of IL-2 inhibited IgG1 a
nd IgE production by Nb-inoculated mice. To understand the mechanism o
f this IL-2-mediated inhibition, we used an in vitro IgG1 and IgE indu
ction system. B cells from Nb-inoculated mice displayed an increase in
the capacity of IL-2 to inhibit lipopolysaccharide (LPS) plus IL-4-dr
iven IgE and IgG1 production, indicating that B cells expressing IL-PR
P are highly sensitive to IL-2. This inhibition was principally depend
ent upon the direct action of IL-2 on a cells. However, partial abolit
ion of IL-2 inhibitory action by anti-IFN-gamma treatment suggested th
at endogenous IFN-gamma released from IL-2-stimulated cells was also i
nvolved in this IL-2-mediated IgE and IgG1 inhibition. Northern blot a
nalysis demonstrated that IL-2 inhibited IL-4 induction of germline an
d productive C-epsilon transcripts in LPS-stimulated a cells. Digestio
n-circularization polymerase chain reaction analysis revealed IL-2 inh
ibited IL-4 induction of s mu-s gamma 1 rearrangement in LPS-stimulate
d B cells.