RESISTANCE IN A LABORATORY POPULATION OF CULEX-QUINQUEFASCIATUS (DIPTERA, CULICIDAE) TO BACILLUS-SPHAERICUS BINARY TOXIN IS DUE TO A CHANGEIN THE RECEPTOR ON MIDGUT BRUSH-BORDER MEMBRANES
C. Nielsenleroux et al., RESISTANCE IN A LABORATORY POPULATION OF CULEX-QUINQUEFASCIATUS (DIPTERA, CULICIDAE) TO BACILLUS-SPHAERICUS BINARY TOXIN IS DUE TO A CHANGEIN THE RECEPTOR ON MIDGUT BRUSH-BORDER MEMBRANES, European journal of biochemistry, 228(1), 1995, pp. 206-210
Direct binding experiments with isolated brush border membrane fractio
ns (BBMF) from larvae of a susceptible laboratory strain of Culex quin
quefasciatus Say, indicated the presence of a single class of Bacillus
sphaericus binary toxin receptors. The dissociation constant (K-d) wa
s approximately 11 nM and the maximum binding capacity (B-max) approxi
mately 8 pmol/mg BBMF protein. Similar binding experiments with a fiel
d population of C. quinquefasciatus that had been selected in the labo
ratory to more than 100000-fold resistance to B. sphaericus binary tox
in failed to reveal the presence of any specific binding. Thus this re
sistant strain had lost the functional receptor for B. sphaericus toxi
n. The binding characteristics of BBMF from the F-1 larval progeny (su
sceptible femalesXresistant males) were very close to those of the par
ental susceptible strain, consistent with the resistance being recessi
ve.