Objective: To improve culture methods for the detection of group B str
eptococcus colonization. Methods: This study prospectively compared th
e standard culture medium, a blood agar plate, to a selective culture
medium, Todd Hewitt broth with antibiotics, and compared vaginal cultu
re with rectal culture at the first prenatal exam. Results: Of the 383
vaginal swabs received for evaluation of the two culture media, 78 (2
0.4%) were positive for group B streptococcus. The detection rates of
the blood agar plate method and the Todd Hewitt broth with antibiotics
were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with a
ntibiotics, an additional 94 patients were cultured vaginally and rect
ally. Twenty-nine (30.9%) had positive cultures. The rate of detection
was 58.6% for the vaginal culture, 89.7% for the rectal culture, and
100% for both culture sites combined.Conclusion: These data indicate t
hat culture detection of group B streptococcus can be improved by usin
g both a selective broth medium and a dual vaginal and rectal culture.