AMINO OXIDASE FROM TRIGONELLA-FOENUM-GRAECUM SEEDLINGS

Amine oxidase was isolated from eight-day-old Trigonella foenum-graecu m seedlings grown in the dark, using rivanol lactate and ammonium sulp hate fractionations, heat denaturation and hydroxylapatite chromatogra phy. The yellow enzyme is a monomer M(r) of 80 000 with pI 6.8, A(max) at 280 nm and a shoulder at 415 nm. Substrate specificity and inhibit or interactions were similar to those of pea amine oxidase. The N-term inal of the enzyme was blocked and no reaction was observed with antib ody against amine oxidase either from Escherichia coli or Micrococcus luteus.