Confocal laser scanning microscopy was used to visualize the radioauto
graph at the light-microscope level. Confocal reflectance images from
developed silver grains were overlaid with the differential interferen
ce contrast image of the tissue. With this technique, a well-focused r
adioautograph can be taken of both tissue sections and all of the silv
er grains dispersed in the thick emulsion layer. Further application o
f this microscopy is discussed.