MICROSATELLITE-BASED PARENTAGE CENTRAL IN CATTLE

As a new approach to parentage control we developed two multiplex coam plification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite p olymorphisms were visualized by automated fluorescence detection on th e Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis soft ware. Allele frequency data were determined from 238 animals. Thirty-f ive bovine parentage control cases not solvable by conventional blood typing could be solved.