SELECTIVE DETECTION OF H-1-NMR RESONANCES OF CHN GROUPS USING A HETERONUCLEAR MAXIMUM-QUANTUM FILTER AND PULSED-FIELD GRADIENTS

A number of approaches are described for the provision of separate one -dimensional H-1 MMR spectra of CH, CH2, and CH3 groups utilizing the natural-abundance C-13 spins and based upon the selection of the maxim um multiple-quantum coherences of the various groups, This sequence is termed edited maximum-quantum proton spectroscopy (MAXY) spectroscopy , The replacement of phase cycling with the application of z magnetic field gradient pulses is also demonstrated, The editing approach is de monstrated using the H-1 NMR spectrum of dexamethasone in DMSO-d(6) so lution, Extension to a complex mixture biofluid is exemplified by the CH3-only H-1 NMR spectrum of human seminal plasma. This aid to the ass ignment of endogenous metabolite resonances is demonstrated to result in dramatic spectral simplification, (C) 1995 Academic Press, Inc.