M. Jorissen et A. Bessems, NORMAL CILIARY BEAT FREQUENCY AFTER CILIOGENESIS IN NASAL EPITHELIAL-CELLS CULTURED SEQUENTIALLY AS MONOLAYER AND IN SUSPENSION, Acta oto-laryngologica, 115(1), 1995, pp. 66-70
Ciliary beat Frequency (CBF) was measured at room temperature in nasal
epithelial cells from 10 control individuals at multiple time points
during sequential monolayer-suspension culture. CBF was 8.4 +/- 1.6 Hz
in the biopsy and decreased in monolayer culture to approximately 6 H
z. During monolayer culture the interindividual variability was increa
sed (3.0 Hz), but not the intercellular and intracellular variability.
Immediately after ciliogenesis in suspension culture, CBF was approxi
mately 8 Hz, with a low interindividual variability. After 3 weeks in
suspension culture CBF was 8.6 +/- 0.9 Hz. There was no difference in
intercellular and intracellular variability of CBF in the different cu
lture conditions. Ciliogenesis in the sequential monolayer-suspension
culture results in quantitative normal ciliary activity, with low vari
ability. The most important parameter of ciliary activity that changes
during ciliary deterioration and ciliogenesis is the CBF itself.