EVALUATION OF INTRACELLULAR CRYOPROTECTANT CONCENTRATION BEFORE FREEZING OF GOAT MATURE OOCYTE

Authors
LEGAL F GASQUI P RENARD JP
Citation
F. Legal et al., EVALUATION OF INTRACELLULAR CRYOPROTECTANT CONCENTRATION BEFORE FREEZING OF GOAT MATURE OOCYTE, Cryo-letters, 16(1), 1995, pp. 3-12
Citations number
19
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
16
Issue
1
Year of publication
1995
Pages
3 - 12
Database
ISI
SICI code
0143-2044(1995)16:1<3:EOICCB>2.0.ZU;2-#
Abstract
The present study was carried out to investigate the permeability of g oat oocytes to water, glycerol, ethylene glycol and propanediol. Perme ability was studied with a dynamic approach and was checked for its te mperature dependence. Our modelling of osmotic behaviour allowed us to maximize the intracellular concentrations of cryoprotectants during a minimal period of exposure before freezing.