CRYOPRESERVATION OF RAT PANCREATIC-ISLETS IN THE PRESENCE OF ETHYLENE-GLYCOL

Authors
SAKONJU I TAURA Y TAKAGI M SUZUKI T TAKIMOTO K NAKAICHI M NAKAMA S
Citation
I. Sakonju et al., CRYOPRESERVATION OF RAT PANCREATIC-ISLETS IN THE PRESENCE OF ETHYLENE-GLYCOL, Cryo-letters, 16(1), 1995, pp. 21-30
Citations number
19
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
16
Issue
1
Year of publication
1995
Pages
21 - 30
Database
ISI
SICI code
0143-2044(1995)16:1<21:CORPIT>2.0.ZU;2-Z
Abstract
Isolated rat pancreatic islets were cryopreserved in the presence of e thylene glycol at a slow cooling rate (-0.3 degrees C/min) without usi ng either stepwise addition or sucrose-mediated dilution methods. The glucose-stimulated insulin release of the islets cryopreserved with et hylene glycol did not differ from that of islets cryopreserved with di methyl sulfoxide. These findings indicate that ethylene glycol is usef ul as a new cryoprotectant for rat islets.