MONOCLONAL-ANTIBODIES TO A 70-KD CYTOKININ-BINDING PROTEIN FROM ETIOLATED MAIZE SEEDLINGS

Authors
ZAGRANICHNAYA TK BROVKO FA SHEPELYAKOVSKAYA AO BOZIEV KM LIPKIN VM
Citation
Tk. Zagranichnaya et al., MONOCLONAL-ANTIBODIES TO A 70-KD CYTOKININ-BINDING PROTEIN FROM ETIOLATED MAIZE SEEDLINGS, Russian journal of plant physiology, 44(1), 1997, pp. 1-5
Citations number
12
Categorie Soggetti
Plant Sciences
Journal title
Russian journal of plant physiology → ACNP
ISSN journal
10214437
Volume
44
Issue
1
Year of publication
1997
Pages
1 - 5
Database
ISI
SICI code
1021-4437(1997)44:1<1:MTA7CP>2.0.ZU;2-K
Abstract
Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) fr om etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity towar d CBP-70 and devoid of cross-reactivity with other soluble proteins fr om etiolated maize seedlings were isolated and characterized. A protoc ol for CBP-70 isolation including affinity chromatography on immobiliz ed monoclonal antibodies is developed.