MORPHOGENETIC MANIFESTATIONS OF THE EXPRESSION OF THE BACTERIAL IPT GENE IN REGENERATED TOBACCO PLANTS IN-VITRO

The capacities for regeneration, callus formation, and organogenesis w ere studied in tobacco (Nicotiana tabacum L.) plants, both wild-type a nd transformed with the active ipt gene of agrobacterium Transgenic pl ants carrying the ipt gene were capable of forming calli only on mediu m supplemented with 2,4-D and kinetin. However, further callus growth did not depend on phytohormone presence. The regenerated transgenic pl ants had short stems with numerous leaves; their roots were initiated three-five days earlier than in the wildtype regenerated plants. Morph ological traits of transformed regenerants are probably conditioned by their specific hormonal system.