THE QUATERNARY STRUCTURE OF THE ADENOVIRUS-12 EARLY REGION 1B 54K PROTEIN

The quaternary structure of the adenovirus early region 1B 54K protein has been examined under denaturing and nondenaturing conditions. In t he presence of SDS the protein has a strong tendency to form disulfide -linked high-molecular-weight polymers. Under nondenaturing, but reduc ing, conditions the in vitro-translated 54K polypeptide forms oligomer s (probably tetramers) of molecular weight approximately 2 X 10(5). Af ter subcellular fractionation of Ad12 early region 1-transformed cells , the 54K E1B protein present in the cytoplasm had a molecular weight similar to that determined for the in vitro-translated material. Howev er, two populations of the viral protein could be distinguished in the nucleus - one of a size similar to that seen in the cytoplasm and the other of appreciably higher molecular weight (approximately 2 x 10(6) ). No difference in migration pattern was observed after treatment of the nuclear extract with DNase I or RNase. A proportion of the Ad12 E1 B 54K protein in both the high- and the low-molecular-weight populatio ns in the nucleus was found to form a complex with p53, and it is ther efore concluded that the very high molecular weight derives from inter action with an, as yet, unidentified component. (C) 1995 Academic Pres s, Inc.