A DESCRIPTION OF A NEW DR ALLELE, DRB1(ASTERISK)1113

We have discovered a previously unpublished HLA-DRB1 allele, observed in a patient (SE), his mother, and one sibling. The undefined allele g ave sporadic positive reactions with sera in the DR52-associated group . SSOPH analysis utilizing both generic and group specific primers and probes also gave ambiguous results. SE typed clearly as a DRB10301 ( paternal allele) but the DNA from SE also bound probes specific for DR B114 and DRB1*11. Sequencing revealed that the undefined allele was s imilar to a DRB114 allele with a segment of sequence found in DRB1*11 alleles. The patient was MLC reactive with donors who express DRB103 01, 1401 and *0301, *11 and was nonreactive solely to DRB1*0301 (Dw3) homozygous typing cells.