Ym. Kwon et al., THE EFFECT OF SYNTHETIC LYSINE AND METHIONINE ON IN-VITRO PROTEIN-SYNTHESIS OF WEANING PIGS, Nutrition research, 15(3), 1995, pp. 429-437
This study was conducted to examine the effects of synthetic lysine an
d methionine in pig diets on protein synthetic activity of liver tissu
e. Weaning pigs were fed 16 % crude protein diets supplemented with 0,
0.1, 0.2 and 0.4 % solid or liquid lysine and 18 % crude protein diet
s as control in experiment 1, and fed 15 % crude protein diets supplem
ented with three levels of lysine-methionine (2:1 ratio) and 18 % crud
e protein diets in experiment 2. Protein synthesis was estimated by me
asuring the amount of incorporated [H-3]-lysine into protein in liver
acinar cell culture. Retained protein from liver tissue of pigs was si
gnificantly increased by supplementation of lysine, and solid form of
lysine was more effective than liquid form. Also, the retained protein
was significantly increased by supplementation of lysine together wit
h methionine. Ln conclusion, the 2 % protein sparing effect in acinar
cell culture of liver tissue was obtained by supplementation of the 0.
2 % lysine and supplementation of lysine together with methionine was
more effective to compensate the decreased protein synthetic activity
by low protein level.