DOSIMETRY OF IRRADIATION MODELS - THE 96-WELL CLONOGENIC-ASSAY FOR TESTING RADIOSENSITIVITY OF CELL-LINES

Radiation experiments with cells in single cell suspension in test tub es and on 96-well plates were carried out and compared, The cells orig inated from cell lines established from carcinomas of the boor of the mouth and from endometrial carcinoma, Two irradiation models were cons tructed, Both models allowed the absorbed doses; to the cells to be ad ministered with a high accuracy in both experimental settings (better than 5.0%). These irradiation models were compared on cancer cell line s with dissimilar inherent radiation sensitivity and histologic type ( UM-SCC-I resistant, UM-SCC-14A sensitive, and UT-EC-2B highly sensitiv e); various radiation doses were used, The fractions of surviving cell s as a function of radiation dose were compared: there was no signific ant difference between cells irradiated in test tubes and cells irradi ated in 96-well plates, Thus, if the absorbed doses in cells suspended in a tube and in a plate were the same, the survival was similar rega rdless of the type of irradiation model.