DONOR ORIGIN OF A POSTTRANSPLANT LIVER ALLOGRAFT MALIGNANCY IDENTIFIED BY FLUORESCENCE IN-SITU HYBRIDIZATION FOR THE Y-CHROMOSOME AND DNA GENOTYPING

Posttransplantation malignancy in the allograft is a rare complication of orthotopic liver transplantation. In the described case, an abnorm al T-tube cholangiogram, performed 6 months after orthotopic liver tra nsplantation between a male donor and a female recipient, prompted nee dle liver biopsy. A moderately differentiated adenocarcinoma was found . Fluorescence in situ hybridization for the Y chromosome indicated ma le origin of malignancy. Donor-related disease was confirmed by compar ative DNA analysis of genomic sequences from the donor liver, associat ed tumor, and recipient peripheral blood. Results of these investigati ons qualified the recipient for a second liver transplant.