A SENSITIVE NOVEL STAINING AGENT FOR THE RESOLVED PROTEINS ON PAGE

A more sensitive, stable and fast-reacting dye was isolated from the b ark of Ratanjot by a simple solvent extraction procedure at high pH. T he dye produced sharp, clear distinct stable bands of known proteins w hen resolved on polyacrylamide gel electrophoresis (PAGE) using sodium dodesyl sulfate (SDS), showing a rapid and strong affinity with prote in molecules. Not only did proteins from peanut, separately stained wi th the new dye and Coomassie Brilliant Blue R-250, produce rapid compa rable results, but also the protein bands were easily visualized and w ere less diffusible with clearer boundaries in the case of the new sta ining agent. (C) Munksgaard 1995.