R. Ali et al., A SENSITIVE NOVEL STAINING AGENT FOR THE RESOLVED PROTEINS ON PAGE, International journal of peptide & protein research, 45(2), 1995, pp. 97-99
A more sensitive, stable and fast-reacting dye was isolated from the b
ark of Ratanjot by a simple solvent extraction procedure at high pH. T
he dye produced sharp, clear distinct stable bands of known proteins w
hen resolved on polyacrylamide gel electrophoresis (PAGE) using sodium
dodesyl sulfate (SDS), showing a rapid and strong affinity with prote
in molecules. Not only did proteins from peanut, separately stained wi
th the new dye and Coomassie Brilliant Blue R-250, produce rapid compa
rable results, but also the protein bands were easily visualized and w
ere less diffusible with clearer boundaries in the case of the new sta
ining agent. (C) Munksgaard 1995.