EFFECT OF VARIOUS BUD INDUCTION TREATMENTS ON ELONGATION AND ROOTING OF ADVENTITIOUS SHOOTS OF CANARY-ISLAND PINE (PINUS-CANARIENSIS)

Three-day-old cotyledonary explants of Pinus canariensis were subjecte d to 30 induction treatments using half-strength Bornman's medium cont aining various combinations of N-6-benzyladenine, zeatin, kinetin and 2-isopentenyl-adenine. The highest numbers of buds were obtained with 10 mu M 6-benzyladenine, but both kinetin and zeatin influenced shoot elongation. Shoots were maintained on half-strength Schenk and Hildebr andt medium with 2% sucrose and 0.05% activated charcoal. For rooting, shoots were pulsed for 4 h in a 100 mu M indole-3-butyric acid aqueou s solution (pH 4.2-4.5), and planted in peat:vermiculite:perlite (1:1: 1). After 8 weeks, the numbers of rooted shoots were similar for most treatments. Therefore, the bud induction treatments did not significan tly influence rooting of adventitious shoots of Canary Island pine.