FACTORS AFFECTING THE IN-VITRO RAPID GERMINATION OF TAXUS EMBRYOS ANDTHE EVALUATION OF TAXOL CONTENT IN THE PLANTLETS

Authors
ZHIRI A JAZIRI M HOMES J VANHAELEN M SHIMOMURA K
Citation
A. Zhiri et al., FACTORS AFFECTING THE IN-VITRO RAPID GERMINATION OF TAXUS EMBRYOS ANDTHE EVALUATION OF TAXOL CONTENT IN THE PLANTLETS, Plant cell, tissue and organ culture, 39(3), 1994, pp. 261-263
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
39
Issue
3
Year of publication
1994
Pages
261 - 263
Database
ISI
SICI code
0167-6857(1994)39:3<261:FATIRG>2.0.ZU;2-Q
Abstract
A simple and efficient in vitro method for breaking the dormancy of Ta xus baccata L. cv. Stricta seeds was investigated. The highest rate of germination (100%) of embryos isolated from seeds, which had been was hed with running tap water for 7 days, was obtained after 7 days of cu lture on modified Murashige and Skoog or Helier media. The taxol equiv alent content in 2-month-old Taxus plantlets was investigated using an ti-taxol polyclonal antibodies. The results showed that the taxol equi valent content varied, depending on Taxus species and on the individua ls in the same taxon.