REGULATION OF HIV-1 GAG PROTEIN SUBCELLULAR TARGETING BY PROTEIN-KINASE-C

The human immunodeficiency virus type 1 internal structural protein pr ecursor, p55, and its corresponding matrix proteolytic fragment, p17, are phosphorylated at Ser(111) by protein kinase C. COS-7 cells transf ected with plasmids encoding either the wild-type or Ser(111) --> Ala mutated human immunodeficiency virus type 1 gag gene matrix domain pro teins were treated with phorbol 12-myristate 13-acetate (PMA), and the phosphorylation of the expressed p17 proteins was examined by radioim munoprecipitation, SDS-polyacrylamide gel electrophoresis, and autorad iography, PMA treatment of transfected cells resulted in a 4-5-fold in crease in wild-type p17 (but not mutated p17) phosphorylation; however , mutated p17 exhibited a low basal level of phosphorylation that was not affected by PMA, suggesting that additional sites were phosphoryla ted, PMA treatment of cells expressing wild-type p17 produced a dramat ic shift in the localization of p17 from the cytosol to the membrane f raction within 8-15 min, followed by a slow quantitative dissociation of p17 back into the cytosol by 90 min, The cytosol-to-membrane transl ocation was dependent on N-myristoylated p17 since cells expressing p1 7 with a Gly(2) --> Ala mutation did not localize to the membrane, PMA also failed to induce the translocation of fully N-myristoylated Ser( 111) --> Ala p17, suggesting that p17 phosphorylation at Ser(111) was responsible for membrane association, This conclusion was confirmed by the finding of phosphorylated wild-type p17 in the membrane fraction only after PMA treatment, These results suggest that a ''myristoyl-pro tein switch'' regulates the reversible membrane targeting of p17 by pr otein kinase C-mediated phosphorylation. This signal may provide a mec hanism for the cellular regulation of virus development through modula tion of gag protein-related developmental steps such as capsid targeti ng, assembly, encapsidation, budding, and maturation.