PRINS AS A METHOD FOR RAPID CHROMOSOMAL LABELING ON HUMAN SPERMATOZOA

Direct in situ labeling of human spermatozoa was performed using the P RINS method. This technique is based on annealing of specific oligonuc leotide primers, and subsequent primer extension by a Taq DNA polymera se. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1-hr reaction. The method was successf ully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detectin g aneuploidies. (C) 1995 Wiley-Liss, Inc.