Wm. Dunne et W. Wang, CLONAL DISSEMINATION AND COLONY MORPHOTYPE VARIATION OF VANCOMYCIN-RESISTANT ENTEROCOCCUS-FAECIUM ISOLATES IN METROPOLITAN DETROIT, MICHIGAN, Journal of clinical microbiology, 35(2), 1997, pp. 388-392
Thirty-two isolates of vancomycin-resistant Enterococcus faecium (VRE)
recovered from 25 patients hospitalized at six hospitals in the metro
politan Detroit, Mich., area over a 32-month period were examined for
relatedness by repetitive-sequence PCR (rep-PCR), All isolates were sh
own to carry the vanA gene by PCR. The rep-PCR patterns generated from
each isolate showed that the first three VRE isolates obtained from h
ospital A between June 1992 and February 1994 were distinct strains. T
hereafter, all VRE isolates originating from hospital A and those coll
ected from five other area hospitals had identical rep-PCR patterns. O
n detailed examination, subcultures of 25 of the 32 VRE isolates produ
ced two distinct colony types characterized phenotypically by a rough
and a smooth appearance, respectively, Both colony types retained the
vanA locus and the rep-PCR pattern of the primary isolate. These data
suggest that a single strain of VRE with the capacity to produce two c
olonial variants has been disseminated to several Detroit-area hospita
ls, The clinical significance of the colonial morphotypes is unclear.