A NOVEL WESTERN-BLOT TEST CONTAINING BOTH VIRAL AND RECOMBINANT PROTEINS FOR ANTICYTOMEGALOVIRUS IMMUNOGLOBULIN-M DETECTION

Authors
LAZZAROTTO T MAINE GT DALMONTE P RIPALTI A LANDINI MP
Citation
T. Lazzarotto et al., A NOVEL WESTERN-BLOT TEST CONTAINING BOTH VIRAL AND RECOMBINANT PROTEINS FOR ANTICYTOMEGALOVIRUS IMMUNOGLOBULIN-M DETECTION, Journal of clinical microbiology, 35(2), 1997, pp. 393-397
Citations number
28
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
2
Year of publication
1997
Pages
393 - 397
Database
ISI
SICI code
0095-1137(1997)35:2<393:ANWTCB>2.0.ZU;2-4
Abstract
We devised a novel Western blot (WB) test for anti-human cytomegalovir us (HCMV) immunoglobulin M (IgM) detection which contains viral struct ural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the most immunogenic nonstructural protei n p52 (ppUL44), This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV i nfection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-speci fic IgM.