A horse,vith no prior clinical history of equine piroplasmosis tested
negative for Babesia caballi and Babesia equi in the complement fixati
on test before importation into the United States from France. After 5
years in residence in the United States, the animal tested serologica
lly positive for B. equi by the complement fixation test, the immunofl
uorescent antibody test, and Western blot analysis. The carrier status
of the horse was confirmed by culture of B. equi parasites. In vitro
culture offers an efficient and comparatively inexpensive method to de
termine the carrier status of horses suspected of harboring B. equi.