A MULTIWELL ASSAY FOR INHIBITORS OF PHOSPHATIDYLINOSITOL-3-KINASE ANDTHE IDENTIFICATION OF NATURAL PRODUCT INHIBITORS

Authors
FREW T POWIS G BERGGREN M ABRAHAM RT ASHENDEL CL ZALKOW LH HUDSON C QAZIA S GRUSZECKAKOWALIK E MERRIMAN R BONJOUKLIAN R
Citation
T. Frew et al., A MULTIWELL ASSAY FOR INHIBITORS OF PHOSPHATIDYLINOSITOL-3-KINASE ANDTHE IDENTIFICATION OF NATURAL PRODUCT INHIBITORS, Anticancer research, 14(6B), 1994, pp. 2425-2428
Citations number
26
Categorie Soggetti
Oncology
Journal title
Anticancer research → ACNP
ISSN journal
02507005
Volume
14
Issue
6B
Year of publication
1994
Pages
2425 - 2428
Database
ISI
SICI code
0250-7005(1994)14:6B<2425:AMAFIO>2.0.ZU;2-M
Abstract
A convenient and reliable multisample assay for the screening of inhib itors of the growth factor signalling enzyme phosphatidylinositol-3-ki nase (PtdIns-3-K) has been developed Four natural product inhibitors o f Ptdlns-3-K have been identified with IC50 values for hypericin 0.18 mu M, emodin 3.3 mu M, asperuloside 2.0 mu M and uttronin A 1.1 mu M.