EXPRESSION AND ALTERATIONS OF CD66 ANTIGEN BY INTERFERON-GAMMA IN HUMAN BREAST-CARCINOMA CELL-LINES

Monoclonal antibodies of the CD66/67 panel from the 5th Workshop on Le ukocyte Antigens bound (as determined by flow cytometry) io the cell s urface of human breast carcinoma cell lines BT-20 and MDA-MB-468. The molecular weight of antigenic polypeptides recognized by the CD66 mono clonal antibody F34-187 differed between the two examined breast carci noma lines as follows: 50kDa, 95 kDa and 130 kDa polypeptides were exp ressed on both BT-20 and MCF-7 cell lines, while the major 160-180 kDa polypeptide was found only in MDA-MB-468 cells. IFN-gamma, all-trans retinoic acid and phorbol ester (TPA) induced up-regulation of CD66 an tigen(s) recognized by the monoclonal antibody F34-187 (as determined by flow cytometry). Different alterations of CD66 antigenic polypeptid es recognized by F34-187 monoclonal antibody, induced by interferon-ga mma and indentified by immunoblotting, were found on BT-20, MCF-7 and MDA-MB-468 breast carcinoma cell lines.