2-DIMENSIONAL GEL ANALYSIS OF P53-MEDIATED CHANGES IN PROTEIN EXPRESSION

The p53 gene product suppresses both tumor cell growth and the cellula r transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many g ene expression events nle involved in p53-mediated growth arrest and a poptosis. Two dimensional gel electrophoresis was employed to investig ate changes in whole-cell protein expression in p53-null H1299 cells t hat were transfected with a recombinant adenovirus expressing wild- ty pe p53 at high efficiency. The two-dimensional gel analysis was restri cted to proteins ranging in mass fram 12,000 to 80,000 daltons. A tota l of 17 proteins were induced and one was repressed within 16 h of exp ression of exogenous wild-type p53 in H1299 cells. These results indic ate that p53-mediated growth suppression involves a complex an ay of g ene expression events.