COMPARISON OF GROWTH-FACTOR RECEPTORS AND METABOLIC CHARACTERISTICS OF SATELLITE CELLS DERIVED FROM THE BICEPS FEMORIS AND PECTORALIS MAJORMUSCLES OF THE TURKEY

Myogenic satellite cells were isolated from the turkey pectoralis majo r (PM), a muscle composed largely of white fibers, and the biceps femo ris (BF), a muscle composed largely of red fibers, and their propertie s were compared in culture. Satellite cells derived from the PM and BF muscles exhibited differences in metabolic parameters, growth factor receptor characteristics, and mitogenic responses. PM satellite cells exhibited greater responsiveness to platelet-derived growth factor (PD GF), and the PDGF receptor on these cells had a higher affinity toward ligand compared to BF cells (P < 0.05). Protein synthesis, protein de gradation, and glucose uptake rates were higher in BF satellite cell c ultures (P < 0.05), correlating with previously reported in vivo measu rements using red and white muscle fibers. (C) 1997 Academic Press