STRAIN-SPECIFIC AND SEX-SPECIFIC DIFFERENCES IN THE GLUTATHIONE-S-TRANSFERASE CLASS-PI IN THE MOUSE EXAMINED BY GRADIENT ELUTION OF THE GLUTATHIONE-AFFINITY MATRIX AND REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A gradient elution with glutathione (GSH) from a GSH-Sepharose 6B affi nity column separated the hepatic mouse glutathione S-transferases (GS T) to the alpha-, mu- and pi-classes. The GST-dependent conjugation of atrazine and glutathione was catalyzled by a pi-class GST. The pi- an d mu-classes were both identified by their respective specific substra tes, and after reverse-phase HPLC, by N-terminal analysis of 19-35 of the amino acids. The alpha-class GST was associated with a high seleni um-independent GSH peroxidase activity and the purified protein had a N-blocked terminal. Strain related differences in the pi-class GST of the CD-1, C57BL/6, DBA/2 and Swiss-Webster males were observed by Phas tGel electrophoresis of the GSH affinity chromatograph separated fract ions, reverse phase HPLC and by N-terminal amino acid sequence analysi s.