SPECIFICITY OF AN ESTERASE (XYLD) FROM PSEUDOMONAS-FLUORESCENS SUBSP CELLULOSA

Authors
FAULDS CB RALET MC WILLIAMSON G HAZLEWOOD GP GILBERT HJ
Citation
Cb. Faulds et al., SPECIFICITY OF AN ESTERASE (XYLD) FROM PSEUDOMONAS-FLUORESCENS SUBSP CELLULOSA, Biochimica et biophysica acta (G). General subjects, 1243(2), 1995, pp. 265-269
Citations number
19
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta (G). General subjects → ACNP
ISSN journal
03044165
Volume
1243
Issue
2
Year of publication
1995
Pages
265 - 269
Database
ISI
SICI code
0304-4165(1995)1243:2<265:SOAE(F>2.0.ZU;2-9
Abstract
Activity of an esterase from Pseudomonas fluorescens subsp, cellulosa (XYLD) on an insoluble feruloylated hemicellulose substrate (de-starch ed wheat bran) was dependent on the source of added endo-xylanase. The esterase exhibited high selectivity for the nature, position of linka ge and size of the feruloylated oligosaccharides generated by hydrolys is of the hemicellulose. Increased affinity of XYLD with increasing si ze of the oligosaccharide substrate suggests that optimal activity is observed on substrates with at least 4 sugars.