Dv. Kuprash et al., HOMODIMER OF P50(NF-KAPPA-B1) DOES NOT INTRODUCE A SUBSTANTIAL DIRECTED BEND INTO DNA ACCORDING TO 3 DIFFERENT EXPERIMENTAL ASSAYS, Nucleic acids research, 23(3), 1995, pp. 427-433
Transcription factors can distort the conformation of the DNA double h
elix upon binding to their target sites. Previously, studies utilizing
circular permutation-electrophoretic mobility shift assay suggested t
hat the homodimer of p50 (NF kappa B1), canonical NF-kappa B (p65-p50)
, as well as several non-canonical NF-kappa B/Rel complexes, may induc
e substantial DNA bending at the binding site. Here we have applied th
ree additional experimental approaches, helical phasing analysis, mini
circle binding and cyclization kinetics, and conclude that the homodim
er of p50 introduces virtually no directed bend into the consensus kap
pa B sequences GGGACTTTCC or GGGAATTCCC.