THE DISTRIBUTION OF SEROTYPE-SPECIFIC PLASMIDS AMONG DIFFERENT SUBGROUPS OF STRAINS OF SALMONELLA-ENTERICA SEROTYPE ENTERITIDIS - CHARACTERIZATION OF MOLECULAR VARIANTS BY RESTRICTION ENZYME FRAGMENTATION PATTERNS
Sc. Rankin et al., THE DISTRIBUTION OF SEROTYPE-SPECIFIC PLASMIDS AMONG DIFFERENT SUBGROUPS OF STRAINS OF SALMONELLA-ENTERICA SEROTYPE ENTERITIDIS - CHARACTERIZATION OF MOLECULAR VARIANTS BY RESTRICTION ENZYME FRAGMENTATION PATTERNS, Epidemiology and infection, 114(1), 1995, pp. 25-40
Pour hundred and thirty-four isolates of Salmonella enterica serotype
Enteritidis were studied. They were grouped into five subsets defined
by either the collection criteria or the parameter which formed the ba
sis for subsequent analysis. Seventy-seven per cent harboured the sero
type-specific plasmid (SSP). In 55% of the isolates this was the sole
plasmid. Molecular variation in the SSP was detected in 17 (5%) of the
isolates on the basis of restriction enzyme fragmentation pattern (RE
FP) analysis using PstI and SmaI. The SSP variants were further charac
terized using additional restriction enzymes chosen to optimize the in
formation content and analysed using a coefficient of similarity. A va
riant SSP designated pOG690 she-wed greater resemblance to the SSP of
Salmonella enterica serotype Typhimurium than Enteritidis; 89% and 68%
respectively for PstI and 79% and 55% respectively for SmaI. In respe
ct-of the PstI data pOG690 shared at least 55 kb of DNA with the Typhi
murium SSP and 37 kb with the SSP of Enteritidis. This variant was ass
ociated with poultry (duck, goose, chicken) and all isolates belonged
to phage type 9b. Other variants were associated with phage types 4, 6
, 6a, 9a, 11, 15 and 24. The epidemiological implications of these res
ults are discussed.