HLA-DR EXPRESSION ON CD8 LYMPHOCYTES FROM GASTRIC-MUCOSA IN UREASE-POSITIVE AND UREASE-NEGATIVE GASTRITIS

We isolated lymphocytes from chronically inflamed gastric mucosa. We a nalysed the expression of lL-2 receptors (CD25), transferin receptors (CD71) and HLA-DR molecules on T lymphocytes by flow cytometric analys is in 16 patients with urcase-positive and in 7 patients with urease-n egative chronic gastritis. In G0, G1 and G2 histotogical type (Sydney classification) of gastritis the number of lymphocytes obtained from t he gastric mucosa biopsies was too low for the flow cytometric analysi s. However, in G3 histological type of chronic gastritis we obtained e nough cells for the flow cytometric analysis in 75 %. We demonstrated a significant increase in HLA-DR expression on CD8 cells from patients with urease-positive gastritis compared to urease-negative gastritis. We also observed a statistically non-significant increase in HLA-DR e xpresssion on CD3 cells, and in CD71 expression on bath CD3 and CD8 ce lls in urease-positive gastritis. However, no difference in CD25 expre ssion was found between the two types of gastritis.