QUANTITATIVE ASSESSMENT OF ANTIMALARIAL ACTIVITIES FROM MALAYSIAN PLASMODIUM-FALCIPARUM ISOLATES BY MODIFIED IN-VITRO MICROTECHNIQUE

Malaysian, TGR (Thailand), and Gambian (West African) Plasmodium falci parum isolates were cultured in vitro by the candle jar method and wer e characterized for their susceptibilities to present antimalarial dru gs by the modified in vitro microtechnique. Results showed that 93 and 47% of the Malaysian isolates were resistant at 50% inhibitory concen trations of 0.1415 to 0.7737 and 0.1025 to 0.1975 mu M, respectively, while the rest were susceptible to chloroquine and cycloguanil at 0.03 76 and 0.0306 to 0.0954 mu M, respectively. All isolates were suscepti ble to mefloquine, quinine, and pyrimethamine at 0.0026 to 0.0172, 0.0 062 to 0.0854, and 0.0149 to 0.0663 mu M, respectively, In contrast, t he Gambian isolate was susceptible to multiple drugs at 0.0024 to 0.02 82 mu M; TGR was resistant to chloroquine at 0.8147 mu M but was susce ptible to mefloquine, quinine, cycloguanil, and pyrimethamine at 0.002 4, 0.0096, 0.0143, and 0.0195 mu M, respectively.