TRANSCRIPTION ACTIVATION AT CLASS-II CRP-DEPENDENT PROMOTERS - THE ROLE OF DIFFERENT ACTIVATING REGIONS

Transcription activation by the Escherichia coli cyclic AMP receptor p rotein (CRP) at Class II promoters is dependent on direct interactions between two surface-exposed activating regions (AR1 and AR2) and two contact sites in RNA polymerase. The effects on transcription activati on of disrupting either AR1 or AR2 have been measured at different Cla ss II promoters, AR2 but not AR1 is essential for activation at all th e Class II promoters that were tested, The effects of single positive control substitutions in AR1 and AR2 vary from one promoter to another : the effects of the different substitutions are contingent on the -35 hexamer sequence, Abortive initiation assays have been used to quanti fy the effects of positive control substitutions in each activating re gion on the kinetics of transcription initiation at the Class II CRP-d ependent promoter pmelRcon. At this promoter, the HL159 substitution i n AR1 results in a defect in the initial binding of RNA polymerase whi lst the KE101 substitution in AR2 reduces the rate of isomerization fr om the closed to the open complex.